Please join us for the Inaugural Triangle SAXS Workshop!
When: Tuesday, May 14, 2013
Where: Duke University
Fitzpatrick CIEMAS Building
Parking is available at the Bryan Center.
8:30 – 9:00 Arrival, Coffee
9:00 – 9:05 Welcome (Bob Rose, Mark Walters, Stefan Zauscher)
9:05 – 9:50 Anton Paar SAXSESSmc2 (Gerd Langenbucher, Anton Paar)
9:50-10:00 Questions and Short Break
10:00-10:45 SAXSLAB Ganesha 300XL (Scott Barton and Karsten Joensen, Saxslab)
10:45-11:15 Questions and Coffee Break
11:15-12:00 Soundbites (short presentations by participants about research and interest in SAXS, Wiersma coordinating)
12:00-1:00 Lunch and Networking
1:00-1:30 Ribbon-Cutting Ceremony
1:30-2:00 SMIF Tour and SAXS Instrumentation (Mark Walters, Gerd Langenbucher, Scott Barton, Karsten Joensen)
2:00-2:10 Questions and Short Break
2:10-3:10 Talk 1 “Introduction to SAXS, Applications and Data Classification” (Peter Worsch, Anton Paar)
3:10-3:30 Coffee Break
3:30-4:30 Talk 2 “Pore Structure and Fluid Sorption in Ordered Mesoporous Silica: New Insight from in-situ Small-Angle X-Ray Scattering” (Gerhard Findenegg, TU-Berlin)
4:30-5:00 Networking and User Group Meeting (discussion about hands-on workshops on May 15-16, 2013)
In an effort, spearheaded by Triangle MRSEC, we received support through the NSF-MRI program for the purchase of Small Angle X-Ray Scattering (SAXS) instrumentation. The state-of-the-art instruments will serve the greater Research Triangle community for research and education, and will be housed in Duke’s Shared Materials Instrumentation Facility (SMIF).
Two, complementary instruments are on order: a slit-collimated SAXSess MC2 from Anton Paar, and a point collimated GANESHA 300XL+ from SAXSlab. The SAXSess MC2 instrument has very high flux at the sample and will be used for scattering from solutions. The GANESHA 300XL+ is fully automated to cover a broad q-range, and will be used for SAXS, MAXS (medium angle X-ray scattering), WAXS (wide angle X-ray scattering), and GISAXS (grazing incidence X-ray scattering) measurements.
More Information: http://saxs.pratt.duke.edu
Rob Ferris just passed his Ph.D. Dissertation defense. Congratulations Rob, a job well done!
Greg presented his poster entitled “VISUALIZING LIPID REACTIVITY OF HIV-1 ANTIGEN AND NEUTRALIZING ANTIBODIES USING ATOMIC FORCE MICROSCOPY” at the 8th annual Center for Aids Research (CFAR) retreat at the Searle Center at Duke University.
Lei just passed her qualifier, and Eric passed his prelim examination.
Greg recently won a Burroughs Wellcome Fund 2012 Collaborative Research Travel Grant. This grant enables Greg to visit our research partner, Dr. Micheal Hirtz at the Karlsruhe Institute of Technology (KIT). Working with Dr. Hirtz we seek to use dip-pen nanolithography (DPN) to write single supported lipid bilayers (SLBs) that mimic components of the native human immunodeficiency virus-1 (HIV-1) lipid-envelope, and to achieve parallel deposition of SLB islands of different composition directly adjacent to each other using DPN.
Prof. Zauscher (PI) and Prof. Munir Alam (Co-PI) received an R21 award from NIH (NIAID) in the amount of $418k for their proposal entitled: HIV-1 Neutralizing Antibody Binding to Viral Membrane Mimics. The research is motivated by the recent identification of broadly neutralizing antibodies from HIV-1 infected subjects, whose antibody attributes include polyreactivity, i.e., the ability to react with both viral and host components, like membrane lipids. The proposed research pairs biophysical techniques with model lipid systems to define and understand the required lipid reactivity of neutralizing antibodies, and has important relevance in HIV-1 vaccine design for the induction of polyreactiv neutralizing antibody responses in humans.
Prof. Zauscher (PI), and Co-Pi’s, Dr. Mark Walters, and Profs. Bob Rose (NCSU) and Sergei Sheiko (UNC) received an award in the amount of $560k from the Major Research Instrumentation program of NSF. The grant is entitled: MRI Consortium: Acquisition of X-Ray Scattering Instrumentation – SAXS/WAXS/GISAXS and provides funding for the acquisition of a Small Angle X-Ray Scattering (SAXS) Instrument.
The SAXS facility will be established at Duke University’s Shared Materials Instrumentation Facility (SMIF) and will serve three major universities in the area: Duke University, North Carolina State University, and University of North Carolina at Chapel Hill. The instrument will serve researchers in materials science, structural biology, polymer and colloidal chemistry, chemical and biomedical engineering, and textiles engineering. The instrument capabilities will be broad to address the different needs of the users, and include wide angle X-ray scattering (WAXS) and grazing incidence small angle X-ray scattering (GISAXS).
Scattering techniques are essential to the research objectives of the newly established NSF Triangle Materials Research Science and Engineering Center (MRSEC), focused on the study of soft matter components for programmable assembly. SAXS also contributes important insights to difficult structural biology problems, including the assembly of protein complexes and conformational flexibility.
Profs. Lingchong You (PI) and Stefan Zauscher (Co-PI) received an award from ONR in the amount of $667k for their proposal entitled: Programming Bacteria for Materials Fabrication. The proposed research represents an innovative application of synthetic biology, which enables the development of novel approaches to fabricate functional materials. As a proof-of-concept demonstration You and Zauscher have chosen the bacterial synthesis of CdS thin films because of the potential for their use in photovoltaic and printed microelectronic devices.
Prof. Zauscher co-chaired the 4th Gordon Research Conference on Biointerface Science in Les Diablerets, Switzerland, May 20 – 25, 2012.
The poster was entitled: “DNA Block Copolymers and Polypeptide DNA Hybrid Copolymers: Towards Engineering of Biologically Inspired Biomaterials.”
Coauthors were Vinalia Tjong, Ashutosh Chilkoti, and Stefan Zauscher.
In April 2012, Prof. Zauscher received the Capers and Marion McDonald Award for Excellence in Teaching and Research from the Pratt School of Engineering at Duke University. In annually recognizing the career achievements of one individual, this award honors all of our engineering faculty, who, over the years, through excellence in both teaching and research have challenged and nurtured our students and strongly contributed to knowledge.
In May 2012 Prof. Zauscher was inducted into the Bass Society of Fellows and was named the Sternberg Family Professor of Mechanical Engineering and Materials Science.
This book, edited by Marcus Textor and Michelle Grandin will become
available soon at Wiley-VCH.
Check out Chapter 1: “Stimulus Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and Applications”
Vinalia Tjong, Jianming Zhang, Ashutosh Chilkoti and Stefan Zauscher
The Triangle MRSEC is studying programmed assembly of soft matter, inventing materials that have never before existed and creating ways to use those materials.
Click here: http://mrsec.duke.edu/reu
REU Projects in the Zauscher Lab:
Temperature-Triggered Micellization of ELP-DNA Block Copolymers
Many efforts have been made to advance new drug delivery system with optimal therapeutic activity while minimize negative side effect. Reversible micellar self-assembly of amphiphilic block copolymer system is becoming an increasingly interesting candidate as a drug delivery vehicle. Here we develop novel block copolymer systems that consist of a polypeptide block with thermally reversible phase transition (Tt), and a hydrophilic, single stranded polydeoxynucleotide (ssPDN) block. The polypeptide block also serves as a macroinitiator for the enzymatic growth of the ssPDN block, using a template-independent polymerase –terminal deoxynucleotidyl transferase (TdT). This REU project will focus on the characterization of the resulting diblock copolymers and their self assembly behavior as a function of block molecular weight and temperature. Students will work in a team environment and will be trained in relevant characterization techniques such as static light scattering, AFM imaging, and small angle X-ray scattering.
Programmed Self-Assembly of Biomacromolecules and Colloidal Particles on Polarized Ferroelectric Thin Films
To date, control over surface charge is largely achieved by chemical patterning and setting the solution pH and ionic strength. These approaches lead to relatively low lateral resolution and surfaces of usually fixed charge. We are currently developing a new approach in which the surface charge density can be switched and controlled with tens of nanometer lateral resolution. Such surfaces have significant promise for detection and sensing applications as they should 1) enable control over the conformation of proteins and polymers at interfaces, 2) enable control over the hydrophobicity/hydrophilicity at the solid/liquid interface, 3) enable control over surface reactivity, and 4) enable the capture and release of drugs and other biological materials onto/from device surfaces. Our approach relies on the high surface charge density that can be achieved by imprinting local surface charge states into programmable ferroelectric thin films, such as lead zirconium titanate (PZT). This in turn can be harnessed for directing the localized self-assembly of biomacromolecules and colloidal particles on the surface. This research is materials science oriented and involves characterization by scanning electron microscopy (SEM), AFM, and ferroelectric capacitance measurements. The project also entails the nanoscale encoding of the polarization of the ferroelectric film locally using the AFM and subsequent characterization of the surface charge pattern and its interaction with proteins, organic molecules and colloidal particles in the immediate vicinity of the surface.
Jan 17, 2012, Prof. Zauscher Presents Invited Lecture at Bio-Inspired Engineering Symposium at Harvard
Lecture: Design and Use of Surface-Tethered (Bio)Macromolecular Nanostructures for Nanomechanical Sensing and Novel Detection and Amplification Platforms
In January 2012, Dr. Zauscher was promoted to Full Professor.
Please consider attending the 2012 Biointerface Science Gordon Research Conference (GRC). The GRC will be held in Les Diablerets, Switzerland, May 20-25, 2012.
(1) Responsive Bio-Interfaces (Sunday PM)
|Discussion Leader: Atul Parikh (Chemical Engineering, UC Davis, USA)|
|Bo Liedberg (Material Science and Engineering, Nanyang Technological University, Singapore)|
|Brent S. Sumerlin (Chemistry, Southern Methodist University, Dallas, USA)|
|(2) Interfacial Architectures (Monday AM)|
|Discussion Leader: Ashutosh Chilkoti (Biomedical Engineering, Duke University, Durham, USA)|
|Curtis W. Frank (Chemical Engineering, Stanford University, USA)|
|Jürgen Rühe (Department of Microsystems Engineering, University of Freiburg, Germany)|
|Jean-Pierre Majoral (Chemistry, CNRS, Toulouse, France)|
|(3) Modeling of Biointerfaces (Monday PM)|
|Discussion Leader: Michael Grunze (Physical Chemistry, University of Heidelberg, Germany)|
|Roland Netz (Physics, Free University of Berlin, Germany)|
|Yaroslava G. Yingling (Materials Science and Engineering, NC State University, Raleigh, USA)|
|(4) Nanoscopic Systems and Interfaces (Tuesday AM)|
|Discussion Leader: Uwe Sleytr (NanoBiotechnology, University of Natural Resources, Vienna, Austria)|
|Zhenan Bao (Chemical Engineering, Stanford University, USA)|
|Erik Reimhult (NanoBiotechnology, University of Natural Resources, Vienna, Austria)|
|Stefan Diez (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany)|
|(5) Bio-Active Interfaces (Tuesday PM)|
|Discussion Leader: Janos Vörös (Biomedical Engineering, ETH Zurich, Switzerland)|
|Gero Decher (Institut Charles Sadron, CNRS, Strasbourg, France)|
|Rigoberto Advincula (Chemistry, University of Houston, USA)|
|(6) Biointerfaces and Devices (Wednesday AM)|
|Discussion Leader: Robert Clark (School of Engineering and Applied Sciences, Rochester, USA)|
|Sabine Szunerits (Chemistry, CNRS, University of Lille, France)|
|Catherine Klapperich (Biomedical Engineering, Boston University, USA)|
|Fabio Biscarini (Institute for Nanostructured Materials, Bologna, Italy)|
|(7) Membranes (Wednesday PM)|
|Discussion Leader: Steve Boxer (Chemistry, Stanford University, USA)|
|Steve Evans (Physics, University of Leeds, United Kingdom)|
|Alain Brisson (Biology, CNRS, University of Bordeaux, France)|
|(8) Cells and Cellular Structures I (Thursday AM)|
|Discussion Leader: Willi Jahnen-Dechent (Cell and Molecular Biology, RWTH Aachen, Germany)|
|Kevin Healy (Bioengineering, UC Berkeley, USA)|
|Kam Leong (Biomedical Engineering, Duke University, Durham, USA)|
|Horst Vogel (Physical Chemistry, EPF Lausanne, Switzerland)|
|(9) Cells and Cellular Structures II (Thursday PM)|
|Discussion Leader: Annette Brunsen (Chemistry, TU-Darmstadt, Germany)|
|Ali Miserez (School of Materials Science and Engineering, Nanyang Technological University, Singapore)|
|Dan Morse (Molecular Biology, UC Santa Barbara, USA)|
Ferroelectric Thin Films in Fluidic Environments: A New Interface for Sensing and Manipulation of Matter
Robert Ferris, Benjamin Yellen and Stefan Zauscher
Congratulations to Greg Hardy and Yee Lam, their paper on “Screening interactions between HIV-1 neutralizing antibodies and model lipid surfaces” was accepted for publication in the Journal of Immunological Methods.
Congratulations to Rob Ferris. The concept paper on “Ferroelectric Thin Films in Aqueous Environments: A New Interface for Sensing and Manipulation of Matter” was accepted for publication in the journal SMALL.
Lei will present her research at the 58th AVS International Symposium & Exhibition in Nashville, TN, October 28-November 4, 2011.